Hepatitis B virus (HBV) infection is a major public health priority. In the present study, a lateral flow strip combined with the recombinase polymerase amplification (LF-RPA) assay was developed and evaluated for rapid HBV detection. A primer/probe pair targeting the conserved region of the HBV genome was designed and applied to the LF-RPA. TheRPA was achieved at the isothermal temperature of 39℃ for 30 min, and the RPA products were detected using the LF test. DNA extraction, RPA reaction and endpoint detection will take about 70 min.
Microplastics pollution in aquatic ecosystems has aroused increasing global concern, leading to an explosive growth of studies regarding microplastics published in the past few years. To date, there is still a lack of standardized methodologies used for the detection of microplastics within environmental samples, thus hampering comparison of the reported data.