Climate change and population growth generates a decrease in water availability around the world which can compromise the maintenance of sustainable agriculture. Thus, treated wastewater (TWW) became an alternative to minimize water shortage. However, this may indirectly affect the soil's microbial properties. In this study different soils irrigated for 0, 1, 8 and 20 years with TWW were sampled and from the east central region of Tunisia.
Over the last two decades, there has been growing interest on the effects of agricultural practices on soil biology in Europe. As soil biota are known to fluctuate throughout the season and as agro-environmental conditions may influence the effect of agricultural practices on soil organisms, conclusions cannot be drawn from a single study. Therefore, integrating the results of many studies in order to identify general trends is required. The main objective of this study was to investigate how soil biota are affected by repeated applications of organic amendments (i.e.
Previous studies already demonstrated that biochar addition reduces nitrogen (N) leaching in soil, but little information is available about its effects on N leaching and bacterial community structure under the application of organic N. This study investigated the effects of corn-straw biochar under the application of urea (250 kg N ha-1) in layered soil columns. The PCR-amplified partial 16S rRNA genes in soil were sequenced before and after biochar treatment in order to assess the change of bacterial diversity and community structure utilizing the Illumina technology.
