Hepatitis B virus (HBV) infection is a major public health priority. In the present study, a lateral flow strip combined with the recombinase polymerase amplification (LF-RPA) assay was developed and evaluated for rapid HBV detection. A primer/probe pair targeting the conserved region of the HBV genome was designed and applied to the LF-RPA. TheRPA was achieved at the isothermal temperature of 39℃ for 30 min, and the RPA products were detected using the LF test. DNA extraction, RPA reaction and endpoint detection will take about 70 min.
The clown anemonefish (Amphiprion ocellaris) is a common model species in studies assessing the impact of climate changes on tropical coral fish physiology, metabolism, growth, and stress. However, the basic endocrine principles for the control of food intake and energy homeostasis, under normal and elevated sea temperatures, in this species remain unknown. In this work, we studied food intake and growth in clown anemonefish reared at different temperatures and with different food availability.
Compared to other climate regions of the world, Mediterranean regions are likely to experience more severe effects of climate change as rainfall decreases and temperatures increase. Global climate change models predict a reduction in rainfall and rise in the temperature of rivers in South Africa's Cape Fold Ecoregion (CFE) – a Mediterranean region in the south-west corner of the country.
Fatty alcohols (FAs) have been widely studied as typical phase-change materials for their high latent heat, low undercooling, non-toxicity, and low cost in thermal energy storage applications. The thermal properties, especially the heat capacity, play a vital role in designing-related energy storage techniques. However, there are few studies on the thermal properties of FAs systematically investigated in a wide temperature region, which greatly limit their application in thermal energy storage field.
Elsevier, Molecular and Cellular Endocrinology, Volume 519, 1 January 2021
The ability to maintain a (relatively) stable body temperature in a wide range of thermal environments by use of endogenous heat production is a unique feature of endotherms such as birds. Endothermy is acquired and regulated via various endocrine and molecular pathways, and ultimately allows wide aerial, aquatic, and terrestrial distribution in variable environments. However, due to our changing climate, birds are faced with potential new challenges for thermoregulation, such as more frequent extreme weather events, lower predictability of climate, and increasing mean temperature.
Background: Various retrospective studies have reported on the increase of mortality risk due to higher diurnal temperature range (DTR). This study projects the effect of DTR on future mortality across 445 communities in 20 countries and regions. Methods: DTR-related mortality risk was estimated on the basis of the historical daily time-series of mortality and weather factors from Jan 1, 1985, to Dec 31, 2015, with data for 445 communities across 20 countries and regions, from the Multi-Country Multi-City Collaborative Research Network.
Background: Hepatitis B causes more than 800 000 deaths globally each year. Perinatal infections are a major driver of this burden but can be prevented by vaccination within 24 h of birth. Currently, only 44% of newborn babies in low-income and middle-income countries (LMICs) receive a timely birth dose. We investigated the effects and cost-effectiveness of implementing ambient storage of hepatitis B vaccines under a controlled temperature chain (CTC) protocol and the use of compact prefilled auto-disable (CPAD) devices for community births.
The net ecosystem exchange (NEE) is the difference between ecosystem CO2 assimilation and CO2 losses to the atmosphere. Ecosystem respiration (Reco), the efflux of CO2 from the ecosystem to the atmosphere, includes the soil-to-atmosphere carbon flux (i.e., soil respiration; Rsoil) and aboveground plant respiration. Therefore, Rsoil is a fraction of Reco and theoretically has to be smaller than Reco at daily, seasonal, and annual scales.
It is no secret to anyone living in Beirut or a similar modern city in a semi-arid tropical country in the summer that their home has become a concrete forest and an urban heat island. Old wood or stone houses and their gardens have been replaced by concrete towers and parking lots, in the name of development. The result is searing summer nights, a drastic loss of insect and avian biodiversity, and a large increase in energy usage for interior climate control. These problems are experienced in rapidly developing urban centers worldwide.
Elsevier, Vaccine, Volume 35, 25 May 2017
Background While our previous work has shown that replacing existing vaccines with thermostable vaccines can relieve bottlenecks in vaccine supply chains and thus increase vaccine availability, the question remains whether this benefit would outweigh the additional cost of thermostable formulations. Methods Using HERMES simulation models of the vaccine supply chains for the Republic of Benin, the state of Bihar (India), and Niger, we simulated replacing different existing vaccines with thermostable formulations and determined the resulting clinical and economic impact.