Antiprotozoal activity of medicinal plants used by Iquitos-Nauta road communities in Loreto (Peru)

Elsevier, Journal of Ethnopharmacology, Volume 210, 10 January 2018
Authors: 
Vasquez-Ocmin P., Cojean S., Rengifo E., Suyyagh-Albouz S., Amasifuen Guerra C.A., Pomel S. et al.
Ethnopharmacological relevance In the Peruvian Amazon, the use of medicinal plants is a common practice. However, there is few documented information about the practical aspects of their use and few scientific validation. The starting point for this work was a set of interviews of people living in rural communities from the Peruvian Amazon about their uses of plants. Protozoan diseases are a public health issue in the Amazonian communities, who partly cope with it by using traditional remedies. Validation of these traditional practices contributes to public health care efficiency and may help identify new antiprotozoal compounds. Aims of study to inventory and validate the use of medicinal plants by rural people of Loreto region. Materials and methods Rural mestizos were interviewed about traditional medication of parasite infections with medicinal plants. Ethnopharmacological surveys were undertaken in two villages along Iquitos-Nauta road (Loreto region, Peru), namely 13 de Febrero and El Dorado communities. Forty-six plants were collected according to their traditional use for the treatment of parasitic diseases, 50 ethanolic extracts (different parts for some of the plants) were tested in vitro on Plasmodium falciparum (3D7 sensitive strain and W2 chloroquine resistant strain), Leishmania donovani LV9 strain and Trypanosoma brucei gambiense. Cytotoxic assessment (HUVEC cells) of the active extracts was performed. Two of the most active plants were submitted to preliminary bioguided fractionation to ascertain and explore their activities. Results From the initial plants list, 10 were found to be active on P. falciparum, 15 on L. donovani and 2 on the three parasites. The ethanolic extract from Costus curvibracteatus (Costaceae) leaves and Grias neuberthii (Lecythidaceae) bark showed strong in vitro activity on P. falciparum (sensitive and resistant strain) and L. donovani and moderate activity on T. brucei gambiense. Conclusions The Amazonian forest communities in Peru represents a source of knowledge on the use of medicinal plants. In this work, several extracts with antiprotozoal activity were identified. This work contributes to validate some traditional uses and opens subsequent investigations on active compounds isolation and identification.

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Abrus Arboreus; Abrus Arboreus Extract; Albizia Niopoides; Albizia Niopoides Extract; Alchornea Castaneifolia; Alchornea Castaneifolia Extract; Alchornea Triplinervia; Alchornea Triplinervia Extract; Animal; Animal Cell; Animals; Antiprotozoal Activity; Antiprotozoal Agent; Antiprotozoal Agents; Apeiba Tibourbou; Apeiba Tibourbou Extract; Article; Bauhinia Herrerae; Bauhinia Herrerae Extract; Campsiandra Angustifolia; Campsiandra Angustifolia Extract; Capirona Decorticans; Capirona Decorticans Extract; Cedrela Odorata; Cedrela Odorata Extract; Cephaelis Tomentosa; Cephaelis Tomentosa Extract; Chelonanthus Alatus; Chelonanthus Alatus Extract; Chemistry; Chloroquine; Clarisia Racemosa; Clarisia Racemosa Extract; Copaifera Paupera; Copaifera Paupera Extract; Costus Curvibracteatus; Costus Curvibracteatus Extract; Cricetinae; Dracontium Spruceanum; Dracontium Spruceanum Extract; Drug Cytotoxicity; Drug Effect; Drug Sensitivity; Ethnopharmacological Survey; Ethnopharmacology; Female; Grias Neuberthii; Grias Neuberthii Extract; Guarea Guidonia; Guarea Guidonia Extract; Gustavia Angustifolia; Gustavia Angustifolia Extract; HUVEC Cell Line; Hamster; Handroanthus Impetiginosus; Handroanthus Impetiginosus Extract; Handroanthus Serratifolius; Handroanthus Serratifolius Extract; Herbal Medicine; Human; Human Cell; Human Umbilical Vein Endothelial Cells; Humans; In Vitro Study; Inga Sertulifera; Inga Sertulifera Extract; Iriartea Deltoidea; Iriartea Deltoidea Extract; Isolation And Purification; Leishmania; Leishmania Donovani; Macrophage; Maytenus Macrocarpa; Maytenus Macrocarpa Extract; Medicinal Plant; Medicinal Plants; Medicine, Traditional; Miltefosine; Minquartia Guianensis; Minquartia Guianensis Extract; Monocyte; Mouse; Nonhuman; Ormosia Costulata; Ormosia Costulata Extract; Parasitic Sensitivity Tests; Parasitology; Parasitosis; Peru; Peruvian Amazon; Plant Extract; Plant Extracts; Plants, Medicinal; Plasmodium; Plasmodium Falciparum; Pouteria Guianensis; Prieurella Prieurii; Procedures; Protozoal Infection; Protozoan Infections; Questionnaire; Roucheria Columbiana; Rural Population; Simarouba Amara; Sloanea Schomburgkii; Stachytarpheta Cayennensis; Surveys And Questionnaires; Swartzia Simplex; Tachigali Polyphylla; Tessaria Integrifolia; Traditional Medicine; Triplaris Weigeltiana; Trypanosoma; Trypanosoma Brucei Gambiense; Umbilical Vein Endothelial Cell; Unclassified Drug; Unindexed Drug; Validation Study; Virola Calophylla; South America