Current Research in Food Science, Volume 1, November 2019,
An Ultra-High Performance Liquid Chromatography combined with Time-of-Flight Mass Spectrometry (UHPLC–ToF-MS) method has been developed for determination of nine mycotoxins, namely aflatoxins (AFB1, AFB2, AFG1 and AFG2), ochratoxin A (OTA), zearalenone (ZEA), toxin T2 (T2) and fumonisins (FB1 and FB2) in maize. The method included a two-step extraction with acetonitrile 80% (v/v). After optimization, the analytical method was validated. The different concentrations tested take in account the Maximum Levels (ML) for maize (Commission Regulation EC no. 1881/2006) and good results for repeatability (%RSDr ≤ 15.4%), reproducibility (%RSDR ≤ 15.9%) and recovery (77.8–110.4%, except for AFG2 at 2 μg/kg which presented a recovery of 73.4%) were achieved. These met the performance criteria imposed by Commission Regulation (EC) no. 401/2006. The method was applied to twenty-two samples from Portuguese producers of maize. Fumonisins were the most frequently detected mycotoxins, but the levels do not exceed those imposed by European legislation.
Acetonitrile; Aflatoxin B1; Aflatoxin B2; Aflatoxin G1; Aflatoxin G2; Article; Cereals; Concentration (parameter); Controlled Study; Fumonisin B1; Fumonisin B2; Maize; Maximum Permissible Dose; Measurement Repeatability; Mycotoxin; Mycotoxins; Nonhuman; Ochratoxin; Portugal; Priority Journal; Process Development; Process Optimization; Reproducibility; T 2 Toxin; Time Of Flight Mass Spectrometry; Time-of-flight Mass Spectrometry; Toxin Analysis; Ultra Performance Liquid Chromatography; Ultra-high Performance Liquid Chromatography; Validation; Validation Study; Zearalenone