Brewer's spent grain (BSG), a protein-rich agro-industrial by-product, was used to develop edible film. The effect of pH and protein concentration on the physical properties of films were studied. While high pH favors the increase in the protein film's solubility, with increased tensile strength, elongation at break, and puncture strength; swelling capacity, water activity, and water vapor permeability (WVP) decreased with increasing pH. Increased concentrations of protein in films resulted in enhanced moisture content, mechanical properties, and water activity. Furthermore, the transparency values of films were increased by increasing pH and decreased by increasing the protein concentration.