This paper supports SDG 3 by analysing the current national action plans of ten ASEAN countries and their alignment with the Global Action Plan on antimicrobial resistance, highlighting five key themes: policy design, implementation tools, monitoring and evaluation, sustainability, and One Health engagement.
A call for research into how planetary health, specifically public health, is linked to climate change and how humans are changing the planet. Climate change and biodiversity loss are impacting human immunity and disease outbreaks
AMPK plays a key role in both diabetes mellitus (DM) and Alzheimer's disease (AD) - targeting this biomolecule and understanding the relationship with DM provides new hope for the treatment of AD
Cystic fibrosis (CF) is caused by a mutation of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene, which disrupts an ion channel involved in hydration maintenance via anion homeostasis. Nearly 5% of CF patients possess one or more copies of the G542X allele, which results in a stop codon at residue 542, preventing full-length CFTR protein synthesis. Identifying small-molecule modulators of mutant CFTR biosynthesis that affect the readthrough of this and other premature termination codons to synthesize a fully functional CFTR protein represents a novel target area of drug discovery. We describe the implementation and integration for large-scale screening of a homogeneous, 1536-well functional G542X-CFTR readthrough assay. The assay uses HEK 293 cells engineered to overexpress the G542X-CFTR mutant, whose functional activity is monitored with a membrane potential dye. Cells are co-incubated with a CFTR amplifier and CFTR corrector to maximize mRNA levels and trafficking of CFTR to the cell surface. Compounds that allow translational readthrough and synthesis of functional CFTR chloride channels are reflected by changes in membrane potential in response to cAMP stimulation with forskolin and CFTR channel potentiation with genistein. Assay statistics yielded Z′ values of 0.69 ± 0.06. As further evidence of its suitability for high-throughput screening, we completed automated screening of approximately 666,000 compounds, identifying 7761 initial hits. Following secondary and tertiary assays, we identified 188 confirmed hit compounds with low and submicromolar potencies. Thus, this approach takes advantage of a phenotypic screen with high-throughput scalability to identify new small-molecule G542X-CFTR readthrough modulators.
Substantial systemic barriers to care exist for Black, Latino, Asian, and other communities of color, as well as low-resourced populations.
Research conducted over the last century has suggested a role for sleep in the processes guiding healthy cognition and development, including memory consolidation.
Children and youth with intellectual and developmental disabilities (I/DD) are at increased risk for obesity, metabolic syndrome, type 2 diabetes, hypertension, hyperlipidemia, and a host of other
In this special article, the authors call for nutrition scientists to collaborate with other sectors, disciplines, and experts to develop a more nuanced understanding of how specific shifts in food systems can have broad impacts on sustainability and to bring about food policy changes in the era of climate disruption and environmental degradation.
People with disabilities, including individuals with intellectual and developmental disabilities (IDD), experience unique circumstances that alter their risk for and experiences of violence and abu
An Editorial in support of SDGs 3, 9, and 13, focusing specifically on how to apply the lessons learned in public health during the COVID-19 pandemic to an equitable and sustainable recovery from the climate crisis.
